ID3EAL Suite
Discovery Solutions
Rapid insights with affordable sequencing at any scale
Benefit
We offer three key benefits: Discovery Services for comprehensive miRNA profiling and biomarker discovery, Research Tools that leverage ID3EAL technology for translational studies, and Early Access to our exciting new product pipeline.
Discovery Services
Comprehensive,
end-to-end miRNA profiling and biomarker discovery services.
Research Tools
Power your basic and translational studies with miRNA profiling products that leverage the ID3EAL technology.
Early Access
Be an early adopter of the newest in our exciting product pipeline and enjoy having a part in shaping a product.
Why microRNA
Important Gene Regulators
miRNAs are small endogenous non-coding RNAs of ~17-25 nucleotides in length conserved across various species. They are essential components of gene regulatory networks and regulate gene expression post-transcriptionally by repressing translation or by triggering the degradation of multiple-target mRNAs.
Multiple Disease Associations
miRNAs are involved in all crucial physiological processes including development, proliferation and differentiation, metabolism, homeostasis and host-viral interactions. Unsurprisingly, their aberrant expression has been implicated in a multitude of diseases, including cancer, cardiovascular, metabolic, neurodegenerative and infectious diseases.
Stability in Biofluids
miRNAs are secreted into circulation, exist stably and readily detected in various biofluids including serum, plasma, urine, saliva, tears and aqueous and vitreous humor.
Ideal for Liquid Biopsy
miRNAs are resistant to the effects of freeze-thaw cycles, large variations in pH and prolonged exposure to room temperature. All these abovementioned characteristics make them promising liquid biopsy biomarkers.
Wide Potential in Clinical Applications
Diagnostic and prognostic tests1,2,5,6
Therapy Selection7
Treatment Monitoring1
Disease Stratification4
Companion Diagnostics3
The ID3EAL Technology
The problem
MicroRNAs (miRNAs) are notoriously difficult to quantify accurately due to their high degree of homology and small size. Their low abundance in circulation further aggravates this difficulty. Interestingly, increasing evidence shows that low abundant miRNA targets in biofluids carry diagnostic value for early detection of diseases.
Hence, there is mounting demand for improved detection and quantification methods, especially since their promise as novel diagnostic and prognostic biomarkers for multiple human diseases is just being realized.
The small size of microRNAs makes detection extremely difficult. Less than 100 miRNAs are robustly detected in biofluids.
Nat Methods. 2014 Aug;11(8):809-15.
Our solution
A unique three primer approach for sensitivity, specificity and robustness.
Uniquely designed stem-loop primer minimizes generation of non-specific cDNA
MicroRNA-specific forward and reverse qPCR primers designed for robust amplification of intended target while preventing amplification of mismatched targets
MicroRNA-specific forward and reverse qPCR primers designed for robust amplification of intended target while preventing amplification of mismatched targets
Video
Watch the animated walkthrough of our technology below
ID3EAL Suite
Exceptional miRNA detection powered by
the class-leading ID3EAL RT-qPCR technology
Research Citation
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