ID3EAL™ PanoramiR
miRNA Knowledge Panel

Minimal Input, Maximum Insight

Targeted miRNA profiling is an effective way to discover biomarkers for disease diagnosis and guiding treatment.

Product Highlights

Minimal Input, Maximum Insight.
The ID3EAL PanoramiR Knowledge Panel is an effective approach for profiling miRNAs important in biological processes, disease mechanisms, or biomarker discovery for clinical applications such as disease diagnosis and prognosis, disease stratification or treatment selection.

> 450 miRNAs Detectable in 200ul of Serum
> 7 Log Dynamic Range
Efficient Discrimination between homologs
Consistent Performance for Difficult Templates
Excellent Reproducibility
Two Hour workflow for the entire process

Benefit

Achieve More with Less

Expertly curated disease associated panel generates meaningful insights.

Highly sensitive and robust technology detects lowly expressed miRNAs with minimal sample input.

Relevance to over 20 diseases including cancers, dementias, cardiovascular disease, liver diseases and more.

Relevance to various sample types including plasma, serum and solid tissues.

Optimal Use Experience

Ready-to-use qPCR plate with pre-spotted primers

Pre-wet lab validated multiplexed RT primer pools and qPCR assays

Reagents pre-aliquoted into experimentally relevant proportions

Spike-in RNA controls included

Semi-automated data analysis template for normalization, fold-change results and data visualizations

Speedy Workflow

RNA-to-Ct in 3 hours to 4 hours with a pre-amplification step to enhance detection with very limiting amounts of starting material.

A Panorama of Pathologies

Category	Examples	microRNA Targets
Cardiovascular	Cardiovascular Disease (e.g. stroke, coronary artery diseases, etc)	140
Metabolic	Diabetes, Liver Cancer	358
Neurological	Alzheimer's Disease, Dementia, Depression, CNS Cancer	114
Respiratory	Lung Disease, Lung Cancer	165
Urological	Kidney Disease, Bladder Cancer	51
Reproductive	Cervical Cancer, Prostate Cancer	319
Gastrointestinal	Stomach Cancer, Colorectal Cancer	224
Hematological	Leukemia, Lymphoma	150

The ID3EAL Technology

The problem

MicroRNAs (miRNAs) are notoriously difficult to quantify accurately due to their high degree of homology and small size. Their low abundance in circulation further aggravates this difficulty. Interestingly, increasing evidence shows that low abundant miRNA targets in biofluids carry diagnostic value for early detection of diseases. Hence, there is mounting demand for improved detection and quantification methods, especially since their promise as novel diagnostic and prognostic biomarkers for multiple human diseases is just being realized.

The small size of microRNAs makes detection extremely difficult. Less than 100 miRNAs are robustly detected in biofluids. Nat Methods. 2014 Aug;11(8):809-15.

Our solution

A unique three primer approach for sensitivity, specificity and robustness. Uniquely designed stem-loop primer minimizes generation of non-specific cDNA

MicroRNA-specific forward and reverse qPCR primers designed for robust amplification of intended target while preventing amplification of mismatched targets

Video

Watch the animated walkthrough of our technology below