WATCHMAKER RNA LIBRARY PREP KITS with Polaris Depletion

Sensitivity meets Speed

Direct 5mC sequencing for multimodal insights

Sensitivity meets Speed

Watchmaker RNA Library Prep
with Polaris® Depletion

Rapidly prepare stranded, whole transcriptome sequencing libraries using Watchmaker RNA Library Prep Kits with Polaris® Depletion. The highly streamlined Polaris Depletion module depletes highly abundant transcripts in human, mouse, and rat samples, providing improved coverage of biologically interesting transcripts, including long non-coding RNAs.

The automation-friendly workflow reduces total turnaround time, hands-on time, and consumable use through a reduction in bead purification and reaction steps. A novel, engineered reverse transcriptase improves the conversion of RNA to cDNA, enabling high-quality performance with 1 ng to 1 µg of total RNA, as well as FFPE-derived RNA.

Key Features and Benefits

Increase sensitivity with clinically relevant samples, including FFPE and inputs as low as 1 ng
Generate high-complexity libraries in under 4.5 hours, including rRNA and globin* depletion
Improve automatability with fewer purifications and reagent additions
Consolidate disparate/niche workflows into one process with broad sample type and input compatibility
Realize the performance benefits of a reverse transcriptase specifically tailored for RNA-seq applications

* Globin depletion in human only

Fusion detection in pediatric oncology — whole transcriptome sequencing chord diagram

Read now: Fusion detection in pediatric oncology:
Insights from whole transcriptome sequencing

Applications

RNA sequencing with degraded samples, such as FFPE
Detection of long non-coding RNAs (lncRNAs)
Gene fusion, splice variant, and isoform detection
Gene expression analysis

Novel transcript discovery
Single nucleotide variant (SNV) detection
Targeted sequencing protocols employing hybridization capture

Process more samples easily with a simple, rapid workflow

Workflow comparison chart showing Watchmaker RNA Library Prep Kit with Polaris Depletion vs competitor kits

Figure 1. Improve automatability and reduce turnaround time. The Watchmaker solution combines and shortens enzymatic steps and has fewer bead purifications in comparison to other commercially available kits, resulting in a highly automatable workflow with significantly reduced hands-on time (up to one hour per plate) and consumable use (up to 1,000 pipette tips per plate).

WATCHMAKER RNA LIBRARY PREP KITS with Polaris Depletion

Watchmaker RNA Library Prepwith Polaris® Depletion

Key Features and Benefits

Applications

Process more samples easily with a simple, rapid workflow

Watchmaker RNA Library Prep
with Polaris® Depletion